Jiang Ling

Jiang Ling, Ph.D., Professor, Doctoral Supervisor
Graduated in 1985 to teach in HZAU, engaged in the breeding of virus-free excellent varieties of citrus, participated in the extremely early varieties Citrus reticulata Banco “Guoqing No. 1” and “Guoqing No. 4” breeding, in 1988 won the Ministry of Agriculture science and technology progress prize (the fifth complete adult) and 1994 won the National Education Commission science and technology progress first prize (the fifth complete adult).
During the period of studying for master’s degree, she carried out the research on virus-free citrus shoot-tip micropropagation grafting and pathogen identification of Huanglongbing (HLB) disease for Guangxi Agricultural Reclamation Bureau. During the period of doctoral study, she carried out the research on high content of medicinal ingredients in Ginkgo biloba by biotechnology and the research on antibodies to hapten dendrobitin. From 2000 to 2002, she went to Japan for senior visiting scholar to study the construction and transformation of papaya coat protein gene, which improved the efficiency of gene transformation. In 2009, she was awarded “China Agricultural Science and Technology Award (9/20)” by the excellent innovation team of the Ministry of Agriculture. In 2010, she participated in the project research of “Research and Genetic Improvement of Citrus Germplasm Resources” and “Citrus Model Cultivation and Storage Technology” led by Academician Deng Xiuxin of the Ministry of Education. Participated in the Hubei Province key project “Application of poly-γ-glutamic acid in fruit trees” (ZDNDD17) project research, long-term cooperation with grassroots, and providing technical support for farmers to increase production and income. From 2010 to 2015, she participated in the public welfare project of the Ministry of Agriculture, and developing light and simplified detoxification technology of citrus. Since 2006, she has hosted 5 National Natural Science Foundation projects (No.30571277, No.30771477, No.3572099, No. 31272146, No.3872064), the molecular mechanism of citrus HLB disease control based on key enzyme of fatty acid metabolism was mainly studied, and the crystal structure data of 4 key pathogenic proteins (ID: 4NK4, 4NK5, 4ZW0, 6KCF) were analyzed by combining structural biology with the study of citrus HLB disease resistance mechanism, and published on protein database (PDB). Based on the structural characteristics, small molecule inhibitors were screened for citrus pretreatment to improve the efficiency of shoot-tip detoxification. She presided over a number of cross-cutting projects on citrus Huanglongbing disease viru-free and pathogen detection. In 2018 and 2021, participated in the national major research and development project “Research and Demonstration of Prevention and control Technology of Citrus HLB Disease Interception Belt and Low Endemic Area in Central China” project (2018YFD0201500), From 2020 to 2022, she participated in the “Research and Development of virus-free plants Breeding Technology for Citrus and Banana” project (No. 2019YFD1001802), and from 2021 to 2024, she was funded by the national major research and development project: “Innovation of pathogenic mechanism and Resistance Germplasm of Citrus HLB Pathogen” (2021YFD1400800-1). After the screening of beneficial microorganisms, the citrus plants treatment can improve the resistance of citrus to HLB disease and improve the virus-free efficiency of citrus. She has published more than 30 professional papers, obtained 6 national invention patents, and registered 5 strains of Chinese collection culture. 2019-2020 were supported by the “three Regions” talent plan of Hubei Province.

[1] 1996.9-1999.6 Huazhong Agricultural University > PhD Agronomy
[2] 1988.9-1991.6 Huazhong Agricultural University > MS Horticulture
[3] 1981.9-1985.6 Hunan Agricultural University > BS Pomology

[1] 2005. 7-present> Huazhong Agricultural University>Professor
[2] 2005.7-1997. 7> Huazhong Agricultural University>Associate Professor
[3] 1997.7-1992.7> Huazhong Agricultural University> Lecturer

1. Yang Y，Wang F，Jiang J, Jiang, L*. Inhibition of Citrus Huanglongbing Disease by Paenibacillus polymyx KN-03 and Analysis with Transcriptome and Microflora. Agronomy 2023, 13, 2958. https://doi.org/10.3390/
2. Jing Nan, Shaoran Zhang, Ping Zhan and Ling Jiang * Discovery of novel GMPS inhibitors of Candidatus Liberibacter asiaticus by structure-based design and enzyme kinetic. Biomolecules Biology 2021, 10, 594. https://doi.org/10.3390/ biology10070594
3. Jing Nan, Shaoran Zhang, Ping Zhan, Ling Jiang*. Evaluation of Bronopol and Disulfiram as Potential Candidatus Liberibacter asiaticus Inosine 5’-Monophosphate Dehydrogenase Inhibitors by Using Molecular Docking and Enzyme kinetic. Molecules. 2020, 25, 2313. doi:10.3390/molecules25102313
4. Nan J, Zhang S, Jiang L*. Antibacterial Potential of Bacillus amyloliquefaciens GJ1 against Citrus Huanglongbing. Plants，2021, 10：
5. Jizhou TANG#, Yuanxi DING#, Jing Nan, Xiangyu YANG, Liang SUN, Xiuyun ZHAO，Ling JIANG*. Transcriptome sequencing and ITRAQ reveal the detoxification mechanism of Bacillus GJ1, apotential biocontrol agent for Huanglongbing PLoS ONE 2018.13(8): e0200427 https://doi.org/10.1371/journal.pone.0200427
6. Tang JZ, Ding YX, Deng L, Nan J, Yang XY, Zhao XY, Jiang, L.*. Response of Candidatus Liberibacter Asiaticus-Infected Citrus Plants to Bacillus amyloliquefaciens GJ1. Austin J Plant Biol. ISSN: 2472-3738 2018; 4(1): 1018. ISSN: 2472-3738 http://www.austinpublishinggroup.com
7. Wang SN, Gao ZQ, Li, Y, Jiang, L*, Dong YH*. Crystal structure of beta-Hydroxyacyl-acyl carrier protein dehydratase (FabZ) from Candidatus asiaticum. Austin Journal of Microbiology. 2015.1(2). ISSN:2471-0296 http://austinpublishinggroup.com/microbiology/onlinefirst.php
8. Jiang L# & Gao Z#, Li Y, Wang S, Dong Y*. Crystal structures and kinetic properties of enoyl-acyl carrier protein reductase I from Candidatus Liberibacter asiaticus. Protein Sci. 2014, 23(4):366-377
9. Pan LJ, Jiang L*. Identification and expression of the WRKY transcription factors of Carica papaya in Response to abiotic and biotic stresses. Mol Biol Rep.2014, 41:1215–1225.
10. Jiang L*. Pan LJ. Identification and Expression of C2H2 Transcription Factor Genes in Carica papaya under Abiotic and Biotic Stresses. Molecular Biology Reports. 2012, 22th of January. DOI: 10.1007/s11033-012-1542-y
11. Jiang L*. Wang J. Liu Z. Wang L. Liu GC, Zhang F. Silencing induced by inverted repeat constructs in protoplasts of Nicotiana benthamiana. Plant Cell, Tissue and Organ Culture. 2010, 100:139-148
12. Zhang Shaoran, Zong Qi, Liu Yang, Jiang Ling *. In situ hybridization analysis of specific nrdB genes of citrus dragon disease pathogens. Chinese Journal of Horticulture, 2023,50 (12): 2689-2700. (in Chinese) doi: 10.16420/j.issn.0513-353x.2022-1095
13. Hu Dangzhen, Xu Aiyuan, Yu Mengyi, Jiang Ling *. Inhibition effect of Bacillus treatment on Huanglong disease of citrus plants [J]. Journal of China Agricultural University, 2021, 26(08): 72-83. (in Chinese)
14. Yang Baomin, JIANG Jialin, Yang Yuehua, ZHONG Cui, LI Shuangru, Jiang Ling *. Analysis of growth promoting effect and differential metabolites of Trichoderma harzii on sugar orange seedlings. Subtropical Agricultural Research, 2002, 18(2):103-112 (in Chinese)
15. Zong Qi, Zhang Shengcai, Fan Guotai, Sun Meng, Jiang Ling *. Effects of poly (γ-glutamate) on traits and GABA of citrus seedlings. Journal of Huazhong Agricultural University. 2020, 39(5): 108-114 (in Chinese)
16. Zong Qi, Zhang Shengcai, Fan Guotai, Huang Yi, Hu Dangzhen, Jiang Ling *. Studies on the promotion of polygamma-glutamic acid growth and mineral element absorption in citrus seedlings. Chinese Fruit and Vegetable. 2020, 40(7): 58-63 (in Chinese)
17. Liu S, Zhou W, Wang L, He S G, Jiang L *. Study on the improvement of yield and quality of Ulick lemon by polygam-glutamic acid, a green synergist. Subtropical Agricultural Research, 2018. 38(7): 6-8 (in Chinese)
18. Fu GS-S, Wei Facai, Sun Liang, Yang Xiangyu, Jiang Ling *. Application of poly-γ-glutamic acid to Pearl plum in Longtan, Guangxi. Subtropical Agricultural Research, 2017.13(1): 19-23 (in Chinese)
19. Huang Su-Chan, Xie Jin-Chang, Yan Xiang, Fang Yi-Wen, Jiang Ling *. Effect of poly-γ-glutamic acid on the quality of Gannan navel orange Newhall. Chinese Fruit and Vegetable, 2017, 37(229): 22-25. (in Chinese)
20. Gong Houqun, Cao Lixin, CHENG Changli, HU Shiquan, Chen Shilin, Xie Heping, ZHONG Jiacheng, JIANG Ling *. Study on the improvement of Kamei quality by polygamma-glutamic acid (PGA), a green synergist. Chinese Fruit and Vegetable, 2017, 37(1): 34-37 (in Chinese)
21. Xie Jin-Chang, Yan Fu-Hua, Shu Ning, Nanjing, Jiang Ling *. Effects of polygamma-glutamic acid on the quality of Ponkan without seeds and jade Pomelo. Subtropical Agricultural Research, 2016,12 (4): 236-241 (in Chinese)
22. Fan Zhang, Ling Jiang *. DsRNA-mediated resistance to papaya ring spot virus (PRSV). Chinese Journal of Plant Sciences, 2011,29 (3):385-391 (in Chinese)
23. Qiao F, Qi P Z, Fu Hui, Zhang P, Wu S, Jiang L *. Study on virulence removal of pathogens from complex infection of citrus decaying disease and leaf fragmentation disease. Journal of Huazhong Agricultural University. 2011, 30(4): 416-421 (in Chinese)
24. Jiang Ling *, Qin Changping, Fu Hui. The Gateway system rapidly constructed the reverse repeat expression vector of papaya ringspot virus gene. Chinese Journal of Agricultural Biotechnology. 2008,16 (3): 526-529 (in Chinese)
25. Jiang Ling *, ZHANG Hongyan, ZHANG Mingtao, Fu Hui. Detection of PRSV in papaya with DIG and alkaline phosphatase labeled cDNA probe. Chinese Journal of Agricultural Biotechnology, 2007,15:496-502 (in Chinese)
26. Jiang Ling, Zhang Wencai, Ke Yun. Ma Xiangtao. Preparation of anti-dendrobiin antibody. Chinese Journal of Immunology. 2000,16 (5) 383-385 (in Chinese)
27. Jiang Ling, Wan Shuyuan, Wang Yinghong. Improvement and research on grafting methods of citrus stem tip. Journal of Huazhong Agricultural University, 1995,14 (4): 381-385 (in Chinese)

1. Huazhong Agricultural University, Inventor: Jiang Ling, DING Zhicheng, ZHANG Shaoran, Zong Qi, Liu Yang, Jiang Jialin. A microbial fermentation complex and its application in the control of citrus yellow dragon disease. Application number: ZL 202310352978.3, publication date: April 4, 2023, approval date: December 6, 2023. Certificate number: 6624355
2. Huazhong Agricultural University: JIANG Ling, Juniper, Yang Yuehua, JIANG Jialin, WANG Fangkui. The invention relates to a test-tube seedling scaffold and its application in virus-removal of citrus stem tip micro-bud grafting. Patent number and application number: 202210427333.7, date of publication: July 28, 2022. Patent number: ZL 2022 1 0427333.7, Date of Authorization announcement: March 31, 2023, Authorization Announcement number: CN 114766359 B Certificate number: 5832588
3. Huazhong Agricultural University: Jiang Ling, Zhan Ping, LIU Zhaoxian, Wang Caiyun. An efficient cultivation method of virus-free yellow rootstock of citrus and its application. Patent number: 201911375848.1, published on December 27, 2019. Authorization date: 2021.6.3
4. Huazhong Agricultural University: Jiang Ling, Ding Yuanxi, Sun Liang, “A method for controlling Citrus Yellow Dragon Disease in potted seedlings”, Patent No: 201610876911.X, Huazhong Agricultural University, published on: 2016.10.09, Authorized on: 2019.11.15
5. Huazhong Agricultural University: Jiang Ling, ZHAO Xiuyun, DING Yuanxi, JIANG Qiankun, Sun Liang, Nanjing. National Invention Patent “Application of a biological control agent in removing citrus Yellow Dragon disease pathogen” 201711375148.3, patent application date 2017.12.29, authorization date: 2021.7.20
6. Huazhong Agricultural University: Jiang Ling, Lu Mingyu. “Technology of citrus scion pretreatment to promote the removal of complex infection pathogen”, national authorized invention patent, application number or patent number :201410132037X. Certificate number: 1892647, authorization date: 2016.1.20